1O54
Crystal structure of SAM-dependent O-methyltransferase (TM0748) from Thermotoga maritima at 1.65 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-06-01 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 64.253, 74.992, 143.701 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.930 - 1.650 |
| R-factor | 0.15918 |
| Rwork | 0.158 |
| R-free | 0.18762 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1i9g |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.409 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (4.2)) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.1.9999) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.930 | 1.740 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Number of reflections | 40334 | |
| <I/σ(I)> | 17.4 | 1.9 |
| Completeness [%] | 95.9 | 78.8 |
| Redundancy | 4.7 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION,SITTING DROP,NANODROP | 5 | 293 | 40% MPD, 0.1M sodium acetate trihydrate, pH 5.0, VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 293K |
