1O51

Crystal structure of a putative PII-like signaling protein (TM0021) from Thermotoga maritima at 2.50 A resolution

Replaces:  1O2C

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-BM
Synchrotron site	APS
Beamline	19-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2002-10-27
Detector	APS-1
Wavelength(s)	0.97914, 0.97934
Spacegroup name	P 43 3 2
Unit cell lengths	100.110, 100.110, 100.110
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	70.710 * - 2.500
R-factor	0.19
Rwork	0.184
R-free	0.22900
Structure solution method	SAD
RMSD bond length	0.018
RMSD bond angle	1.780 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	REFMAC (5.1.9999)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	70.710 *	2.590
High resolution limit [Å]	2.500	2.500
Rmerge	0.085 *	0.499 *
Total number of observations	126573 *
Number of reflections	6360 *
<I/σ(I)>	70.05	10.64
Completeness [%]	100.0	100
Redundancy	43.83	0.57

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	7.9 *	293	2M ammonium sulfate, 0.1M tri-sodium citrate dihydrate pH 5.6, 0.2M potassium sodium tartrate tetrahydrate, VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 293K, pH 5.60

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	Tris	20 (mM)	pH7.9
2	1	drop		150 (mM)
3	1	drop	TCEP	0.25 (mM)
4	1	drop	protein	17 (mg/ml)
5	1	reservoir	ammonium sulfate	2.0 (M)
6	1	reservoir	sodium potassium tartrate	0.2 (M)
7	1	reservoir	citrate	0.1 (M)	pH5.6