Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7B |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.834 |
| Spacegroup name | P 1 2 1 |
| Unit cell lengths | 92.340, 65.850, 116.440 |
| Unit cell angles | 90.00, 107.56, 90.00 |
Refinement procedure
| Resolution | 18.000 - 2.050 |
| Rwork | 0.213 |
| R-free | 0.26000 |
| Structure solution method | MAD |
| RMSD bond length | 0.007 * |
| RMSD bond angle | 1.400 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 18.000 | 2.090 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rmerge | 0.053 * | 0.242 * |
| Number of reflections | 77715 | 3382 * |
| <I/σ(I)> | 10.8 | 1.8 |
| Completeness [%] | 92.7 | 81.2 |
| Redundancy | 3.6 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 * | 20 * | Ivens, A., (2001) Eur.J.Biochem., 268, 2246. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 6-8 (mg/ml) | |
| 2 | 1 | reservoir | HEPES | 0.1 (M) | pH7.0 |
| 3 | 1 | reservoir | PEG1500 | 12-18 (%) |
