1O0F
RNASE A in complex with 3',5'-ADP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1999-11-25 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.5419 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 99.862, 32.694, 71.930 |
Unit cell angles | 90.00, 90.66, 90.00 |
Refinement procedure
Resolution | 29.020 - 1.500 |
R-factor | 0.229 |
Rwork | 0.229 |
R-free | 0.27100 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 1afu |
RMSD bond length | 0.004 |
RMSD bond angle | 23.500 * |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.550 |
High resolution limit [Å] | 1.500 | 1.500 |
Rmerge | 0.062 * | 0.369 * |
Total number of observations | 423949 * | |
Number of reflections | 36948 | |
<I/σ(I)> | 14.9 | |
Completeness [%] | 97.9 | 95.5 |
Redundancy | 11.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 16 * | Leonidas, D.D., (1997) Biochemistry, 36, 5578. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 10 (mg/ml) | |
2 | 1 | drop | sodium citrate | 10 (mM) | |
3 | 1 | drop | PEG4000 | 10 (%) | |
4 | 1 | reservoir | PEG4000 | 20 (%) | |
5 | 1 | reservoir | sodium citrate | 20 (M) | pH5.5 |