1NZK
Crystal Structure of a Multiple Mutant (L44F, L73V, V109L, L111I, C117V) of Human Acidic Fibroblast Growth Factor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 103 |
| Detector technology | CCD |
| Collection date | 2002-10-21 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 96.747, 74.033, 109.085 |
| Unit cell angles | 90.00, 89.98, 90.00 |
Refinement procedure
| Resolution | 29.600 - 1.950 |
| R-factor | 0.196 |
| Rwork | 0.194 |
| R-free | 0.25300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1jt7 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.600 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | TNT |
| Refinement software | TNT |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.600 | 2.000 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.060 | 0.164 |
| Number of reflections | 55365 | |
| <I/σ(I)> | 21.8 | 2.9 |
| Completeness [%] | 97.0 | 100 |
| Redundancy | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298.15 | Sodium Formate, Ammonium Sulfate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.15K |
