1NYL

Unliganded glutaminyl-tRNA synthetase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	41.400, 64.120, 208.880
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 * - 2.400 *
R-factor	0.251
Rwork	0.251
R-free	0.33400 *
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007 *
RMSD bond angle	1.230 *
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000 *
High resolution limit [Å]	2.400 *
Rmerge	0.059 *	0.378 *
Total number of observations	141412 *
Number of reflections	21185 *
Completeness [%]	93.5 *	95.8 *
Redundancy	3.7 *	3.6 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.5 *	290 *	used microseeding *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG1000	10 (%)
2	1	reservoir	PEG8000	10 (%)
3	1	reservoir	sodium acetate	0.1 (M)
4	1	reservoir	AMPCPP	5 (mM)
5	1	reservoir	PIPES	0.1 (M)	pH7.5