1NYK
Crystal Structure of the Rieske protein from Thermus thermophilus
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-04-20 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.7373, 1.3776, 1.7415 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 58.569, 78.267, 136.794 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 24.000 - 1.310 |
R-factor | 0.1592 |
Rwork | 0.158 |
R-free | 0.18900 * |
Structure solution method | MAD |
RMSD bond length | 0.012 * |
RMSD bond angle | 2.280 * |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((SCALA)) |
Refinement software | SHELXL-97 |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 * | 1.370 |
High resolution limit [Å] | 1.300 | 1.300 * |
Rmerge | 0.057 * | 0.431 * |
Total number of observations | 319817 * | |
Number of reflections | 76407 * | |
Completeness [%] | 98.8 | 93.3 * |
Redundancy | 4.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 297 | Peg 4000, Tris, magnesium chloride, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 297K |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 297 | Peg 4000, Tris, magnesium chloride, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 297K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 20 (mg/ml) | |
2 | 1 | reservoir | PEG4000 | 25 (%) | |
3 | 1 | reservoir | Tris | 100 (mM) | pH8.5 |
4 | 1 | reservoir | 0.1-0.4 (M) | ||
5 | 1 | reservoir | 10000-11000nm |