1NYI
Crosslinked Hammerhead Ribozyme Initial State
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-12-01 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.15 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 65.639, 65.639, 137.475 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 19.750 * - 2.850 |
R-factor | 0.21 * |
Rwork | 0.203 |
R-free | 0.23000 * |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | urx 057 |
RMSD bond length | 0.010 |
RMSD bond angle | 2.007 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | CNS |
Refinement software | REFMAC (5.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 100.000 * | 2.923 |
High resolution limit [Å] | 2.850 | 2.850 |
Rmerge | 0.072 | 0.349 |
Total number of observations | 8413 * | |
Number of reflections | 7544 | |
<I/σ(I)> | 13.8 | 2.2 |
Completeness [%] | 99.3 * | 99.3 |
Redundancy | 5.2 | 5.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5 * | 16 * | 1.8 M Lithium sulphate and 50 mM sodium cacodylate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 20.0K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | LiSO4 | ||
2 | 1 | 1 | sodium cacodylate | ||
3 | 1 | 2 | LiSO4 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | ribozyme | 1 (mM) | |
2 | 1 | drop | sodium acetate | 50 (mM) | pH5.0 |
3 | 1 | drop | 1.8 (M) | ||
4 | 1 | reservoir | sodium acetate | 50 (mM) | pH5.0 |
5 | 1 | reservoir | 1.8 (M) | ||
6 | 1 | reservoir | EDTA | 1.0 (mM) |