1NYE
Crystal structure of OsmC from E. coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-06-14 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 49.529, 90.291, 112.677 |
| Unit cell angles | 90.00, 93.90, 90.00 |
Refinement procedure
| Resolution | 19.960 - 2.400 |
| R-factor | 0.218 |
| Rwork | 0.218 |
| R-free | 0.27700 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.500 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.600 | 2.440 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Number of reflections | 38395 | |
| <I/σ(I)> | 25.6 | 2.2 |
| Completeness [%] | 98.2 | 97.9 |
| Redundancy | 3.8 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 295 | 0.2M magnesium formate, 20%PEG3350, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
