1NXP
MicArec pH4.5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX14.2 |
| Synchrotron site | SRS |
| Beamline | PX14.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-11-14 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.978 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 79.395, 92.694, 36.775 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 31.470 - 1.820 |
| R-factor | 0.18373 |
| Rwork | 0.179 |
| R-free | 0.23040 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1nxt |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.911 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.1.27) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.470 | 1.867 |
| High resolution limit [Å] | 1.820 | 1.820 |
| Rmerge | 0.072 * | 0.345 * |
| Total number of observations | 199669 * | |
| Number of reflections | 11155 | |
| Completeness [%] | 98.6 | 99.3 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 * | 20 * | PEG 300, Sodium acetate, pH 4.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | Tris-HCl | 25 (mM) | |
| 2 | 1 | drop | 300 (mM) | pH7.5 | |
| 3 | 1 | drop | protein | 10 (mg/ml) | |
| 4 | 1 | reservoir | PEG300 | 30 (%(v/v)) | |
| 5 | 1 | reservoir | Tris-HCl | 0.1 (M) | pH7.0 |
