1NXH
X-RAY STRUCTURE: NORTHEAST STRUCTURAL GENOMICS CONSORTIUM TARGET TT87
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-11-19 |
| Detector | CUSTOM-MADE |
| Wavelength(s) | 0.97924 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 80.223, 80.223, 106.246 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.900 - 2.800 |
| R-factor | 0.226 |
| Rwork | 0.226 |
| R-free | 0.31900 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SnB |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.970 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Number of reflections | 8628 | |
| Completeness [%] | 96.0 | 78 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9.2 | 293 | 0.1M Tris-HCl 9.2, 0.2M Sodium Acetate, 30% PEG 4000, 2% Glycerol , VAPOR DIFFUSION, HANGING DROP, temperature 293K |
