1NWT

Crystal structure of human cartilage gp39 (HC-gp39) in complex with chitopentaose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	OTHER
Temperature [K]	120
Detector technology	IMAGE PLATE
Detector	MAC Science DIP-2030
Spacegroup name	P 43
Unit cell lengths	128.320, 128.320, 108.580
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 * - 2.500
R-factor	0.218
Rwork	0.218
R-free	0.25300
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009 *
RMSD bond angle	1.400 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000 *
High resolution limit [Å]	2.500	2.500
Rmerge	0.114 *	0.626 *
Total number of observations	227637 *
Number of reflections	59975
<I/σ(I)>	10.1	2
Completeness [%]	97.9	96.1
Redundancy	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.2 *		pH 5.1

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	4 (mg/ml)
2	1	drop		1 (M)
3	1	drop	BES	10 (mM)	pH7.2
4	1	reservoir	PEG8000	10 (%)
5	1	reservoir		0.5 (M)
6	1	reservoir	sodium citrate	0.1 (M)	pH5.1