1NVX

Structural evidence for feedback activation by RasGTP of the Ras-specific nucleotide exchange factor SOS

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F1
Synchrotron site	CHESS
Beamline	F1
Temperature [K]	100
Detector technology	CCD
Collection date	2001-01-29
Detector	ADSC QUANTUM 4
Wavelength(s)	0.948
Spacegroup name	I 4 2 2
Unit cell lengths	175.206, 175.206, 234.128
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 3.200
R-factor	0.223
Rwork	0.223
R-free	0.26100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1nvu
RMSD bond length	0.008
RMSD bond angle	21.300 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	3.310
High resolution limit [Å]	3.200	3.200
Rmerge	0.128 *	0.542 *
Total number of observations	255485 *
Number of reflections	29067
<I/σ(I)>	15.7	5.2
Completeness [%]	96.6	100
Redundancy	8.8	9.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	277	2-8% PEG 4000, 100 mM MES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10-20 (mg/ml)
2	1	reservoir	PEG4000	2-8 (%)
3	1	reservoir	MES	100 (mM)	pH6.5