1NVN
Structural Characterisation of the Holliday junction formed by the sequence CCGGTACCGG at 1.8 A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 100 |
| Wavelength(s) | 0.811 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 66.380, 23.790, 37.120 |
| Unit cell angles | 90.00, 110.13, 90.00 |
Refinement procedure
| Resolution | 34.920 - 1.800 |
| R-factor | 0.20872 |
| Rwork | 0.206 |
| R-free | 0.26361 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.731 |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.920 | 2.100 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.060 | 0.200 |
| Number of reflections | 5158 | |
| <I/σ(I)> | 16.78 | 6.06 |
| Completeness [%] | 98.3 | 98.4 |
| Redundancy | 3.71 | 3.73 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 290 | MPD, CaCl2, cacodylate, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 290K |
