1NV8

N5-glutamine methyltransferase, HemK

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2002-06-25
Detector	ADSC QUANTUM 4
Wavelength(s)	1.033
Spacegroup name	C 1 2 1
Unit cell lengths	141.489, 59.062, 85.605
Unit cell angles	90.00, 109.22, 90.00

Refinement procedure

Resolution	20.000 * - 2.200
R-factor	0.18958
Rwork	0.186
R-free	0.25400 *
Structure solution method	MAD
RMSD bond length	0.020 *
RMSD bond angle	1.800 *
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.280
High resolution limit [Å]	2.200	2.200
Rmerge	0.050	0.351
Total number of observations	176619 *
Number of reflections	32346 *
<I/σ(I)>	26	2
Completeness [%]	94.7	66.5
Redundancy	5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	19 *	PEG 3000, NaCitrate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 100K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	14 (mg/ml)
2	1	reservoir	PEG3000	16-20 (%)
3	1	reservoir	sodium citrate trihydrate	100 (mM)	pH5.5