1NU8
Crystal structure of human dipeptidyl peptidase IV (DPP-IV) in complex with Diprotin A (IPI)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 90 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-03-12 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.92 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 65.136, 67.067, 419.635 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 * - 2.500 |
| R-factor | 0.225 |
| Rwork | 0.225 |
| R-free | 0.28200 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.019 * |
| RMSD bond angle | 2.070 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CCP4 |
| Refinement software | REFMAC (5.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 12.000 | 2.600 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.091 * | 0.159 * |
| Total number of observations | 171090 * | |
| Number of reflections | 64208 | |
| Completeness [%] | 97.5 | 99.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | Peg, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | reservoir | PEG3350 | 20-25 (%) | |
| 3 | 1 | reservoir | 200 (mM) | ||
| 4 | 1 | reservoir | Tris | pH8.5 | |
| 5 | 1 | reservoir | glycerol | 15 (%) |
