1NU4
U1A RNA binding domain at 1.8 angstrom resolution reveals a pre-organized C-terminal helix
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-08-15 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 91.819, 91.819, 120.448 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 * - 1.800 |
| R-factor | 0.203 |
| Rwork | 0.203 |
| R-free | 0.23440 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1urn |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.105 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.031 | 0.311 |
| Total number of observations | 70351 * | |
| Number of reflections | 17618 | |
| <I/σ(I)> | 37.6 | 2.46 |
| Completeness [%] | 96.2 | 76.8 |
| Redundancy | 4 | 1.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 * | 298 | Potassium Malonate, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | RNA | 0.15 (mM) | |
| 2 | 1 | drop | protein | 0.22 (mM) | |
| 3 | 1 | drop | potassium cacodylate | 5 (mM) | pH6.5 |
| 4 | 1 | drop | 1.25 (mM) | ||
| 5 | 1 | drop | EDTA | 0.05 (mM) | |
| 6 | 1 | reservoir | sodium malonate | 2.0-2.4 (M) | pH5.0 |
