1NTY
Crystal structure of the first DH/PH domain of Trio to 1.7 A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-04-22 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.92 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 99.540, 99.540, 98.294 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.820 - 1.700 |
| R-factor | 0.195 |
| Rwork | 0.195 |
| R-free | 0.21400 |
| Structure solution method | Molecular Replacement followed by ARP/wARP |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.730 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Number of reflections | 56048 | |
| <I/σ(I)> | 18.8 | 2 |
| Completeness [%] | 90.4 | 55.8 |
| Redundancy | 24.8 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Batch Method. | 7.5 | 277 | DTT, pH 7.5, Batch Method., temperature 277K |
