1NT9
Complete 12-subunit RNA polymerase II
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-11-02 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9185 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 220.400, 391.400, 282.200 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 4.200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1i6h |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 4.350 |
| High resolution limit [Å] | 4.200 | 4.200 |
| Rmerge | 0.093 * | 0.363 |
| Number of reflections | 83431 | 7893 * |
| Completeness [%] | 93.8 | 89.5 |
| Redundancy | 2.9 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 20 * | PEG 20000, ammonium sodium phosphate, sodium chloride, dioxane, DTT, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 4 (mg/ml) | |
| 2 | 1 | reservoir | PEG20000 | 8 (%) | |
| 3 | 1 | reservoir | sodium ammonium phosphate | 360 (mM) | pH6.0 |
| 4 | 1 | reservoir | dioxane | 50 (mM) | |
| 5 | 1 | reservoir | dithiothreitol | 5 (mM) |
