1NT8
Structural Characterisation of the Holliday junction formed by the sequence CCGGTACCGG at 2.00 A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-05-16 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.811 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 65.949, 23.756, 37.204 |
| Unit cell angles | 90.00, 109.98, 90.00 |
Refinement procedure
| Resolution | 34.920 - 2.000 |
| R-factor | 0.24834 |
| Rwork | 0.247 |
| R-free | 0.28262 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.024 |
| RMSD bond angle | 3.768 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.920 | 2.010 |
| High resolution limit [Å] | 1.910 | 1.910 |
| Rmerge | 0.103 | 0.849 |
| Number of reflections | 4276 | |
| <I/σ(I)> | 4.18 | 0.8 |
| Completeness [%] | 97.6 | 97.4 |
| Redundancy | 4 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 290 | MPD, Calcium chloride, cacodylate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
