1NSK

THE CRYSTAL STRUCTURE OF A HUMAN NUCLEOSIDE DIPHOSPHATE KINASE, NM23-H2

Experimental procedure

Detector technology	IMAGE PLATE
Detector	MARRESEARCH
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	118.670, 118.800, 90.190
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 - 2.800
R-factor	0.249
Rwork	0.249
R-free	0.29400
RMSD bond length	0.006
RMSD bond angle	1.500
Data reduction software	MOSFLM
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	25.000
High resolution limit [Å]	2.800
Rmerge	0.087
Number of reflections	29553
Completeness [%]	92.8
Redundancy	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	8.5 *	4 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	Tris-HCl	0.1 (M)
2	1	drop	ammonium sulfate	1.8 (M)
3	1	drop	glycerol	15 (%(w/v))
4	1	reservoir	Tris-HCl	0.1 (M)
5	1	reservoir	ammonium sulfate	2.2 (M)
6	1	reservoir	glycerol	20 (%(w/v))