1NSI

HUMAN INDUCIBLE NITRIC OXIDE SYNTHASE, ZN-BOUND, L-ARG COMPLEX

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	CCD
Collection date	1998-08-15
Detector	ADSC
Spacegroup name	P 43 21 2
Unit cell lengths	187.350, 187.350, 227.490
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.550
R-factor	0.209
Rwork	0.209
R-free	0.24300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	ENOS HEME DOMAIN
RMSD bond length	0.007
RMSD bond angle	23.490 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	X-PLOR (3.8)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.590
High resolution limit [Å]	2.550	2.550
Rmerge	0.052 *	0.488 *
Total number of observations	477621 *
Number of reflections	125717
<I/σ(I)>	12.2	2.3
Completeness [%]	95.9	71.2
Redundancy	3.8	2.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	5	7 *	pH 5.0

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	GSH	2.0 (mM)
3	1	drop	L-Arg	10 (mM)
4	1	reservoir	ammonium sulfate	35 (%sat)
5	1	reservoir	glycerol	30 (%(v/v))
6	1	reservoir	sodium citrate	50 (mM)