1NSF
D2 HEXAMERIZATION DOMAIN OF N-ETHYLMALEIMIDE SENSITIVE FACTOR (NSF)
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL1-5 |
| Synchrotron site | SSRL |
| Beamline | BL1-5 |
| Temperature [K] | 80 |
| Detector technology | CCD |
| Collection date | 1998-03 |
| Detector | ADSC QUANTUM |
| Spacegroup name | P 6 |
| Unit cell lengths | 115.996, 115.996, 44.130 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 500.000 - 1.900 |
| R-factor | 0.224 |
| Rwork | 0.224 |
| R-free | 0.24400 |
| Structure solution method | MAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.260 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS (0.4) |
| Refinement software | CNS (0.4) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 500.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.053 | 0.334 |
| Number of reflections | 50548 | |
| <I/σ(I)> | 33.3 | 4.8 |
| Completeness [%] | 96.5 | 97.5 |
| Redundancy | 6.7 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7 * | 4 * | drop contains equal volume of protein and reservoir solutions * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 15 (mg/ml) | |
| 2 | 1 | drop | HEPES | 20 (mM) | |
| 3 | 1 | drop | 100 (mM) | ||
| 4 | 1 | drop | ADP | 20 (mM) | |
| 5 | 1 | drop | 5 (mM) | ||
| 6 | 1 | drop | glycerol | 2 (%) | |
| 7 | 1 | drop | dithiothreitol | 20 (mM) | |
| 8 | 1 | reservoir | MES | 100 (mM) | |
| 9 | 1 | reservoir | PEG6000 | 0.5 (%) |
