1NSB
THE 2.2 ANGSTROMS RESOLUTION CRYSTAL STRUCTURE OF INFLUENZA B NEURAMINIDASE AND ITS COMPLEX WITH SIALIC ACID
Experimental procedure
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 88.900, 88.900, 222.800 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 7.000 - 2.200 |
| R-factor | 0.148 |
| Rwork | 0.148 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 3.100 |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 2.200 * |
| Rmerge | 0.071 * |
| Total number of observations | 120666 * |
| Number of reflections | 48764 * |
| Completeness [%] | 92.0 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Batch method * | 7.9 * | microseeding * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | protein | 6 (mg/ml) | |
| 2 | 1 | 1 | PEG4000 | 5 (%) | |
| 3 | 1 | 1 | 75 (mM) | ||
| 4 | 1 | 1 | phosphate | 60 (mM) |
