1NR4
High resolution crystal structures of thymus and activation-regulated chemokine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 90 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-08-01 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 |
| Unit cell lengths | 44.350, 56.525, 76.616 |
| Unit cell angles | 69.97, 85.56, 72.74 |
Refinement procedure
| Resolution | 20.000 * - 1.720 |
| R-factor | 0.20115 |
| Rwork | 0.199 |
| R-free | 0.24100 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | RANTES |
| RMSD bond length | 0.029 * |
| RMSD bond angle | 2.500 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 * | 1.780 |
| High resolution limit [Å] | 1.720 | 1.720 |
| Rmerge | 0.075 * | 0.279 * |
| Number of reflections | 65586 | |
| <I/σ(I)> | 12 | 5.1 |
| Completeness [%] | 92.8 | 92.9 * |
| Redundancy | 7 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 * | 285 | 0.16M ammonium sulfate, 0.08M Sodium acetate, 20% PEG 4000, 15% glycerol, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 285K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 25 (mg/ml) | |
| 2 | 1 | reservoir | ammonium acetate | 0.17 (M) | |
| 3 | 1 | reservoir | trisodium citrate | 0.085 (M) | pH5.6 |
| 4 | 1 | reservoir | PEG4000 | 25.5 (%(w/v)) | |
| 5 | 1 | reservoir | glycerol | 15 (%(v/v)) |
