1NQT

Crystal structure of bovine Glutamate dehydrogenase-ADP complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2000-01-01
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	96.460, 171.930, 439.680
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 * - 3.500
R-factor	0.2071
Rwork	0.207
R-free	0.27600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	bovine glutamate dehydrogenase
RMSD bond length	0.016
RMSD bond angle	1.920 *
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000 *	3.660
High resolution limit [Å]	3.500	3.500
Rmerge	0.040 *	0.111 *
Number of reflections	87616
<I/σ(I)>	13.7	3.5
Completeness [%]	70.7 *	49.6 *
Redundancy	2.3 *	1.6 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	6.8	298	sodium phosphate, PEG 8000 sodium azide, sodium chloride, MPD and OBG, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	glycerol suspension	50 (%)
10	1	reservoir	ADP	0.3 (mM)
2	1	drop	sodium phosphate	0.1 (M)	pH6.7-6.8
3	1	drop	protein	10 (mg/ml)
4	1	reservoir	sodium phosphate	0.1 (M)
5	1	reservoir	octyl-beta-glucopyranoside	1 (%(w/v))
6	1	reservoir	MPD	6 (%(v/v))
7	1	reservoir	sodium phosphate	1 (M)
8	1	reservoir	PEG8000	14 (%(w/v))
9	1	reservoir	sodium azide	1 (mM)