1NQS
Structural Characterisation of the Holliday Junction formed by the sequence d(TCGGTACCGA) at 1.97 A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X11 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-05-15 |
Detector | MARRESEARCH |
Wavelength(s) | 0.811 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 65.588, 23.628, 37.223 |
Unit cell angles | 90.00, 111.15, 90.00 |
Refinement procedure
Resolution | 34.710 - 1.970 |
R-factor | 0.22538 |
Rwork | 0.223 |
R-free | 0.27779 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 1.982 |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((SCALA)) |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 34.710 | 2.080 |
High resolution limit [Å] | 1.970 | 1.970 |
Rmerge | 0.070 | 0.296 |
Number of reflections | 3877 | |
<I/σ(I)> | 6.1 | 6.1 |
Completeness [%] | 99.3 | 100 |
Redundancy | 7.6 | 5.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 290 | MPD, calcium chloride, cacodylate, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 290K |