1NPX

STRUCTURE OF NADH PEROXIDASE FROM STREPTOCOCCUS FAECALIS 10C1 REFINED AT 2.16 ANGSTROMS RESOLUTION

Experimental procedure

Spacegroup name	I 2 2 2
Unit cell lengths	77.200, 134.500, 145.900
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	7.000 - 2.160
R-factor	0.177
Rwork	0.177
RMSD bond length	0.015
RMSD bond angle	3.000
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	9999.000 *
High resolution limit [Å]	2.160 *	2.160 *
Rmerge	0.061 *
Number of reflections	37857 *
Completeness [%]	92.0 *	77 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	7 *	20-24 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
10	1	reservoir		3 (mM)
2	1	drop	potasssium phosphate
3	1	drop	EDTA	0.5 (mM)
4	1	drop	dithiothreitol	2 (mM)
5	1	reservoir	potasssium phosphate
6	1	reservoir	EDTA	0.5 (mM)
7	1	reservoir	dithiothreitol	2 (mM)
8	1	reservoir	ammonium sulfate	2.05 (M)
9	1	reservoir	FAD	0.005 (mM)