1NPO

BOVINE NEUROPHYSIN II COMPLEX WITH OXYTOCIN

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH3R
Temperature [K]	295
Detector technology	AREA DETECTOR
Collection date	1990-06-06
Detector	SIEMENS-NICOLET X100
Spacegroup name	P 41 21 2
Unit cell lengths	69.050, 69.050, 113.260
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 3.000
R-factor	0.182
Rwork	0.182
R-free	0.26600
RMSD bond length	0.010
RMSD bond angle	2.790 *
Data reduction software	XENGEN (V. 2.0)
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]		3.180
High resolution limit [Å]	3.000	3.000
Rmerge	0.056	0.253
Total number of observations	38324 *
Number of reflections	5924
<I/σ(I)>	10.6	1.7
Completeness [%]	100.0	100
Redundancy	6.4	5.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Batch method *	6 *	18 *	pH 6.8

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	NP	12.5 (mg/ml)
2	1	1	sodium azide	0.2 (%(w/v))
3	1	1	OT acetate salt		1.7mg
4	1	1	ammonium sulfate		saturated, 0.02ml per well