1NPK

REFINED X-RAY STRUCTURE OF DICTYOSTELIUM NUCLEOSIDE DIPHOSPHATE KINASE AT 1,8 ANGSTROMS RESOLUTION

Experimental procedure

Spacegroup name	P 63 2 2
Unit cell lengths	74.200, 74.200, 105.140
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	8.000 - 1.800
R-factor	0.196
Rwork	0.196
RMSD bond length	0.013
RMSD bond angle	1.280
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
High resolution limit [Å]	1.800 *	1.800 *
Rmerge	0.067 *	0.350 *
Total number of observations	57800 *
Number of reflections	14427 *
Completeness [%]	93.5 *	84 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	8 *	18 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	PEG6000	5 (%)
2	1	drop		20 (mM)
3	1	drop	Tris-HCl	50 (mM)
4	1	reservoir	PEG6000	10 (%)
5	1	reservoir	Tris-HCl	50 (mM)