1NPH
Gelsolin Domains 4-6 in Active, Actin Free Conformation Identifies Sites of Regulatory Calcium Ions
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX14.1 |
| Synchrotron site | SRS |
| Beamline | PX14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-01-31 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.488 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 122.520, 122.520, 82.723 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.000 - 3.000 |
| Rwork | 0.247 |
| R-free | 0.29800 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1DB0 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.330 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 87.700 | 3.160 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.120 * | 0.310 * |
| Number of reflections | 13141 * | |
| <I/σ(I)> | 5.2 | 2.3 |
| Completeness [%] | 99.4 * | |
| Redundancy | 6.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 20 * | PEG 8000, calcium chloride, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | Tris-HCl | 25 (mM) | pH8.0 |
| 2 | 1 | reservoir | PEG8000 | 15 (%(w/v)) | |
| 3 | 1 | reservoir | 0.1 (mM) | ||
| 4 | 1 | drop | protein | 10 (mg/ml) |
