1NPC

THE STRUCTURE OF NEUTRAL PROTEASE FROM BACILLUS CEREUS AT 0.2-NM RESOLUTION

Experimental procedure

Spacegroup name	P 65 2 2
Unit cell lengths	76.500, 76.500, 201.000
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	10.000 - 2.000
R-factor	0.175
RMSD bond length	0.011
RMSD bond angle	2.500
Refinement software	TNT

Data quality characteristics

	Overall
Rmerge	0.219 *
Total number of observations	195223 *
Number of reflections	21168 *
Completeness [%]	88.0 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6 *	0 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	reservoir	PEG6000	18 (%(w/v))
3	1	reservoir	potassium succinate	0.05 (M)
4	1	reservoir	calcium chloride	0.01 (M)