1NO5

Structure of HI0073 from Haemophilus influenzae, the nucleotide binding domain of the HI0073/HI0074 two protein nucleotidyl transferase.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-BM
Synchrotron site	APS
Beamline	17-BM
Temperature [K]	173
Detector technology	CCD
Collection date	2002-04-13
Detector	MARRESEARCH
Wavelength(s)	0.9800
Spacegroup name	P 63
Unit cell lengths	89.560, 89.560, 61.480
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.800 * - 1.800
R-factor	0.201 *
Rwork	0.200
R-free	0.28200
Structure solution method	SAD
RMSD bond length	0.011
RMSD bond angle	0.034
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	SHELXL-97

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.800 *	1.830
High resolution limit [Å]	1.800	1.800
Rmerge	0.028	0.269
Total number of observations	960910 *
Number of reflections	51005
<I/σ(I)>	32.14	5
Completeness [%]	0.0 *	100
Redundancy	6.5	6.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5 *	294	10% 2-Propanol, 0.1 M Sodium cacodylate, 0.2 M Zn(acetate)2, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 294K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	Tris-HCl	20 (mM)	pH7.5
2	1	drop	EDTA	1 (mM)
3	1	drop	protein	15 (mg/ml)
4	1	reservoir	2-propanol	11 (%)
5	1	reservoir	sodium cacodylate	0.1 (M)	pH6.5
6	1	reservoir	zinc acetate	0.2 (M)