1NO4

Crystal Structure of the pre-assembly scaffolding protein gp7 from the double-stranded DNA bacteriophage phi29

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-BM-C
Synchrotron site	APS
Beamline	14-BM-C
Temperature [K]	100
Detector technology	CCD
Collection date	2001-11-17
Detector	ADSC QUANTUM 4
Wavelength(s)	1.000
Spacegroup name	P 21 21 2
Unit cell lengths	114.240, 114.164, 60.297
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	60.300 - 2.200
R-factor	0.239
Rwork	0.239
R-free	0.26200
Structure solution method	MAD
RMSD bond length	0.006
RMSD bond angle	16.400 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	60.300	2.260
High resolution limit [Å]	2.200	2.200
Rmerge	0.068 *	0.251 *
Number of reflections	40285 *
<I/σ(I)>	19.5	5
Completeness [%]	95.7	75.4
Redundancy	20.06

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	6.5	298	4 % PEG 8000, 0.16 M calcium acetate, 0.08 M sodium cacodylate, 20 % glycerol, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	PEG8000	14.4 (%(v/v))
2	1	1	sodium cacodylate	0.08 (M)	pH6.5
3	1	1	cadmium acetate	0.16 (M)
4	1	1	glycerol	20 (%(v/v))
5	1	1	protein	1 (mg/ml)