1NO4
Crystal Structure of the pre-assembly scaffolding protein gp7 from the double-stranded DNA bacteriophage phi29
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-C |
| Synchrotron site | APS |
| Beamline | 14-BM-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-11-17 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 114.240, 114.164, 60.297 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 60.300 - 2.200 |
| R-factor | 0.239 |
| Rwork | 0.239 |
| R-free | 0.26200 |
| Structure solution method | MAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 16.400 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.300 | 2.260 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.068 * | 0.251 * |
| Number of reflections | 40285 * | |
| <I/σ(I)> | 19.5 | 5 |
| Completeness [%] | 95.7 | 75.4 |
| Redundancy | 20.06 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 6.5 | 298 | 4 % PEG 8000, 0.16 M calcium acetate, 0.08 M sodium cacodylate, 20 % glycerol, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | PEG8000 | 14.4 (%(v/v)) | |
| 2 | 1 | 1 | sodium cacodylate | 0.08 (M) | pH6.5 |
| 3 | 1 | 1 | cadmium acetate | 0.16 (M) | |
| 4 | 1 | 1 | glycerol | 20 (%(v/v)) | |
| 5 | 1 | 1 | protein | 1 (mg/ml) |
