1NNJ
Crystal structure Complex between the Lactococcus lactis Fpg and an abasic site containing DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-07-04 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.93 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 92.150, 92.150, 142.530 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.000 - 1.900 |
| Rwork | 0.215 |
| R-free | 0.23600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1kfv |
| RMSD bond length | 0.005 * |
| RMSD bond angle | 1.210 * |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.000 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.074 * | 0.627 * |
| Number of reflections | 56858 | |
| <I/σ(I)> | 6.1 | 1.2 |
| Completeness [%] | 97.6 | 94.8 |
| Redundancy | 11.6 | 9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 * | 298 | citrate, Hepes, Glycerol, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | citrate | ||
| 2 | 1 | 1 | Hepes | ||
| 3 | 1 | 1 | Glycerol | ||
| 4 | 1 | 2 | Glycerol |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5 (mg/ml) | |
| 2 | 1 | reservoir | sodium citrate | 1.4 (M) | |
| 3 | 1 | reservoir | sodium HEPES | 0.1 (M) | pH7.5 |
