1NND

Arginine 116 is Essential for Nucleic Acid Recognition by the Fingers Domain of Moloney Murine Leukemia Virus Reverse Transcriptase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2000-01-15
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	P 21 21 2
Unit cell lengths	38.795, 116.652, 61.652
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.840 - 2.300
R-factor	0.21
Rwork	0.203
R-free	0.27400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1QAI MOLECULE A
RMSD bond length	0.005
RMSD bond angle	1.253 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.050 *	0.130 *
Total number of observations	58875 *
Number of reflections	12471 *
<I/σ(I)>	13.6
Completeness [%]	95.8 *	83.9
Redundancy	9.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	7	20 *	PEG 400, Magnesium Chloride, HEPES, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	0.55 (mM)
2	1	drop	5'-d(CATGCATG)-3'	1.1 (mM)
3	1	drop	PEG400	12-18 (%)
4	1	drop	HEPES	50 (mM)	pH7.0
5	1	drop		5 (mM)
6	1	reservoir	PEG400	2-6 (%)