1NN6
Human Pro-Chymase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-1 |
| Synchrotron site | SSRL |
| Beamline | BL9-1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-03-30 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.08 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 50.688, 55.639, 88.686 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.700 * - 1.750 |
| R-factor | 0.20499 |
| Rwork | 0.203 |
| R-free | 0.24500 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1klt |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.800 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.700 | 1.770 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.040 | 0.690 * |
| Total number of observations | 222007 * | |
| Number of reflections | 27938 * | |
| <I/σ(I)> | 26.8 | 1.7 |
| Completeness [%] | 98.3 | 97.5 |
| Redundancy | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 4 * | 20% Peg 4k, 150mM Na Formate, and 100 mM Tris , pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | 500 (mM) | ||
| 2 | 1 | drop | Bis-Tris | 2 (mM) | pH6.1 |
| 3 | 1 | drop | protein | 5.0 (mg/ml) | |
| 4 | 1 | reservoir | PEG4000 | 20 (%) | |
| 5 | 1 | reservoir | sodium formate | 150 (mM) | |
| 6 | 1 | reservoir | Tris | 100 (mM) | pH8.5 |
