1NLW
Crystal structure of Mad-Max recognizing DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Detector technology | CCD |
| Wavelength(s) | 0.92 |
| Spacegroup name | P 1 |
| Unit cell lengths | 47.346, 56.020, 65.602 |
| Unit cell angles | 88.82, 79.08, 67.11 |
Refinement procedure
| Resolution | 20.000 - 2.000 |
| R-factor | 0.264 |
| Rwork | 0.264 |
| R-free | 0.32400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1nkp |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.940 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | |
| High resolution limit [Å] | 2.000 | |
| Rmerge | 0.052 | |
| Total number of observations | 214612 * | |
| Number of reflections | 35483 | |
| Completeness [%] | 88.8 | 59.9 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 15 * | 20% MPD, 5mM magnesium chloride, 50 mM sodium cacodylate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 288K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | 20% methyl pentane diol | ||
| 2 | 1 | 1 | 5MM magnesium chloride | ||
| 3 | 1 | 1 | 50MM sodium cacodylate pH 6.0 | ||
| 4 | 1 | 2 | methyl pentane diol | ||
| 5 | 1 | 2 | magnesium chloride | ||
| 6 | 1 | 2 | sodium cacodylate pH 6.0 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | MPD | 20 (%) | |
| 2 | 1 | reservoir | 5 (mM) | ||
| 3 | 1 | reservoir | sodium cacodylate | 50 (mM) | pH6.0 |
| 4 | 1 | drop | protein | 10 (mg/ml) |
