1NLT

The crystal structure of Hsp40 Ydj1

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F2
Synchrotron site	CHESS
Beamline	F2
Temperature [K]	100
Detector technology	CCD
Collection date	2002-07-20
Detector	ADSC QUANTUM 4
Wavelength(s)	1.2828,1.2894, 1.244, 1.3237
Spacegroup name	P 32 2 1
Unit cell lengths	55.025, 55.025, 161.870
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	3.000 * - 2.700
R-factor	0.269
Rwork	0.269
R-free	0.29600
Structure solution method	MAD
RMSD bond length	0.008
RMSD bond angle	1.910 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	27.500	2.870
High resolution limit [Å]	2.700	2.700
Rmerge	0.054	0.145 *
Number of reflections	7827
<I/σ(I)>	27.3	8.67
Completeness [%]	95.0 *	73.1
Redundancy	5.22 *	1.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	7	300	PEG 4K, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 300K
1	Vapor diffusion *	7	300	PEG 4K, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 300K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	reservoir	Tris	100 (mM)	pH7.0
3	1	reservoir	ethylene glycol	15 (%)
4	1	reservoir	PEG4000	5 (%)
5	1	drop	MES	10 (mM)	pH6.0
6	1	drop		150 (mM)