1NL5

Engineered High-affinity Maltose-Binding Protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU300
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2002-08-01
Detector	MARRESEARCH
Wavelength(s)	1.54179
Spacegroup name	P 43 21 2
Unit cell lengths	121.590, 121.590, 62.440
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	60.800 - 2.100
R-factor	0.252
Rwork	0.252
R-free	0.29000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1anf
RMSD bond length	0.006
RMSD bond angle	22.100 *
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	60.800	2.180
High resolution limit [Å]	2.100	2.100
Rmerge	0.046 *	0.190
Completeness [%]	99.9	100
Redundancy	7.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.2 *		PEG 3350, Zinc Chloride, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 100K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG5000 MME	18-24 (%)
2	1	reservoir	cacodylate	0.1 (M)	pH6.2
3	1	reservoir	sodium acetate	200 (mM)
4	1	reservoir	zinc acetate	10 (mM)
5	1	reservoir		5 (mM)
6	1	reservoir	maltose	1 (mM)