1NKI
CRYSTAL STRUCTURE OF THE FOSFOMYCIN RESISTANCE PROTEIN A (FOSA) CONTAINING BOUND PHOSPHONOFORMATE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-C |
| Synchrotron site | APS |
| Beamline | 14-BM-C |
| Temperature [K] | 113 |
| Detector technology | CCD |
| Collection date | 2002-06-20 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 54.982, 66.895, 76.723 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 0.950 |
| R-factor | 0.147 |
| R-free | 0.16100 * |
| Structure solution method | AB INITIO |
| Starting model (for MR) | 1lqp |
| RMSD bond length | 0.022 |
| RMSD bond angle | 2.130 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELX |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 0.980 |
| High resolution limit [Å] | 0.950 | 0.950 |
| Rmerge | 0.058 | 0.358 |
| Number of reflections | 154452 | |
| <I/σ(I)> | 26 | 2.28 |
| Completeness [%] | 91.3 | 46.8 |
| Redundancy | 8.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 7 | 298 | Rife, C.L., (2002) J.Am.Chem.Soc., 124, 11001. * |
