1NJI
Structure of chloramphenicol bound to the 50S ribosomal subunit
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2002-01-22 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 211.720, 299.750, 573.430 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 3.000 |
| R-factor | 0.209 |
| Rwork | 0.176 |
| R-free | 0.20100 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1jj2 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 15.600 * |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.970 |
| High resolution limit [Å] | 3.000 * | 3.000 * |
| Rmerge | 0.246 | 0.880 * |
| Number of reflections | 393074 | |
| <I/σ(I)> | 8.88 | 1.5 |
| Completeness [%] | 96.4 * | 96.5 * |
| Redundancy | 5.0 * | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | back-extraction, vapor diffusion * | 7.1 * | 291 | Ban, N., (2000) Science, 289, 905. * |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | KCl | ||
| 10 | 1 | 2 | PEG 6000 | ||
| 2 | 1 | 1 | NH4Cl | ||
| 3 | 1 | 1 | MgCl2 | ||
| 4 | 1 | 1 | sodium acetate | ||
| 5 | 1 | 1 | PEG 6000 | ||
| 6 | 1 | 2 | KCl | ||
| 7 | 1 | 2 | NH4Cl | ||
| 8 | 1 | 2 | MgCl2 | ||
| 9 | 1 | 2 | sodium acetate |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | 1.2 (M) | ||
| 2 | 1 | reservoir | 0.5 (M) | ||
| 3 | 1 | reservoir | potassium acetate | 100 (mM) | |
| 4 | 1 | reservoir | 30 (mM) | ||
| 5 | 1 | reservoir | PEG6000 | 7 (%) | |
| 6 | 1 | reservoir | Tris | 15 (mM) | |
| 7 | 1 | reservoir | MES | 15 (mM) | |
| 8 | 1 | reservoir | 1 (mM) |
