1NIV
MANNOSE-SPECIFIC AGGLUTININ (LECTIN) FROM SNOWDROP (GALANTHUS NIVALIS) BULBS IN COMPLEX WITH MANNOSE-ALPHA 1,3-METHYL-D-MANNOSE
Experimental procedure
| Detector technology | IMAGE PLATE |
| Collection date | 1994-09-09 |
| Detector | RIGAKU |
| Spacegroup name | I 21 3 |
| Unit cell lengths | 138.149, 138.149, 138.149 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 3.000 |
| R-factor | 0.226 * |
| Rwork | 0.227 |
| R-free | 0.26900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | GNA-ALPHA-MEMAN COMPLEX |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.500 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 3.110 | |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.107 | 0.289 |
| Total number of observations | 39125 * | |
| Number of reflections | 8280 | |
| <I/σ(I)> | 9 | 2.9 |
| Completeness [%] | 93.1 | 86.3 |
| Redundancy | 4.7 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 8.5 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | protein | 25 (mg/ml) | |
| 2 | 1 | 1 | ammonium bicarbonate | 0.05 (M) | |
| 3 | 1 | 1 | MeMan-2 | 0.023 (M) |
