1NIK
Wild Type RNA Polymerase II
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-10-31 |
| Detector | ADSC QUANTUM 210 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 224.098, 394.462, 284.343 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.000 - 4.100 |
| R-factor | 0.3335 |
| Rwork | 0.334 |
| R-free | 0.35700 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1I6H WITH DNA AND RNA REMOVED 1GO3 COVERTED TO POLY-ALA MODEL |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 4.250 |
| High resolution limit [Å] | 4.100 | 4.100 |
| Rmerge | 0.108 * | 0.614 * |
| Number of reflections | 96820 * | 9357 * |
| <I/σ(I)> | 5.9 | 1.06 |
| Completeness [%] | 98.8 | 96.6 |
| Redundancy | 3.5 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.5 * | 291 | peg400, peg 6K, NaCl, Dioaxne, ZnCl2, Ammonium phosphate, pH 6.3, VAPOR DIFFUSION, HANGING DROP, temperature 291K, pH 6.30 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 10 | 1 | reservoir | PEG400 | 20-25 (%) | |
| 2 | 1 | drop | Tris-Cl | 5 (mM) | pH7.5 |
| 3 | 1 | drop | ammonium sulfate | 60 (mM) | |
| 4 | 1 | drop | dithiothreitol | 10 (mM) | |
| 5 | 1 | reservoir | ammonium phosphate | 100 (mM) | pH6.3 |
| 6 | 1 | reservoir | 100 (mM) | ||
| 7 | 1 | reservoir | dioxane | 5 (mM) | |
| 8 | 1 | reservoir | 1 (mM) | ||
| 9 | 1 | reservoir | PEG6000 | 5 (%) |
