1NI0
Structure of the Y94F mutant of the restriction endonuclease PvuII
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X13 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X13 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-09-04 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.801 |
| Spacegroup name | P 4 21 2 |
| Unit cell lengths | 176.860, 176.860, 46.340 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.500 |
| R-factor | 0.228 |
| Rwork | 0.228 |
| R-free | 0.29000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1pvu |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.700 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.540 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.074 | 0.454 |
| Number of reflections | 25788 | |
| <I/σ(I)> | 24.3 | 4.6 |
| Completeness [%] | 98.9 | 99.5 |
| Redundancy | 11.3 | 11 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | N/A, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
