1NH9

Crystal Structure of a DNA Binding Protein Mja10b from the hyperthermophile Methanococcus jannaschii

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2002-04-15
Detector	SBC-2
Wavelength(s)	0.9868
Spacegroup name	P 65 2 2
Unit cell lengths	51.506, 51.506, 125.307
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 2.000
Rwork	0.209
R-free	0.26400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1h0x
RMSD bond length	0.009
RMSD bond angle	1.405 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.070
High resolution limit [Å]	2.000	2.000
Rmerge	0.049 *	0.311 *
Total number of observations	91002 *
Number of reflections	6946 *
<I/σ(I)>	21.4	4.2
Completeness [%]	96.6 *	99.1
Redundancy	13.1 *	13.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.25	291	Wang, G.G., (2002) Acta Cryst., D58, 1240. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	reservoir	MPD	40 (%)
3	1	reservoir	Tris-HCl	0.1 (M)	pH8.25