1NH1

Crystal Structure of the Type III Effector AvrB from Pseudomonas syringae.

Experimental procedure

Experimental method	MAD
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	200
Detector technology	IMAGE PLATE
Detector	RIGAKU
Spacegroup name	P 65
Unit cell lengths	125.028, 125.028, 63.246
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	500.000 * - 2.200
Rwork	0.222
R-free	0.27500 *
Structure solution method	MAD
RMSD bond length	0.010 *
RMSD bond angle	1.540 *
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.200
Rmerge	0.066 *	0.493 *
Number of reflections	27892
Completeness [%]	93.4 *	64.6 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.9 *	4 *	VAPOR DIFFUSION, HANGING DROP
1	VAPOR DIFFUSION, HANGING DROP	7.9 *	4 *	VAPOR DIFFUSION, HANGING DROP

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	Tris	20 (mM)	pH7.9
2	1	drop		50 (mM)
3	1	drop	TCEP	0.25 (mM)
4	1	drop	protein	9.5 (mg/ml)
5	1	reservoir	PEG600	40 (%)
6	1	reservoir	CHES	100 (mM)	pH9.5-10.0