1NGW

Chimeric Affinity Matured Fab 7g12 complexed with mesoporphyrin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	CCD
Detector	ADSC QUANTUM 4
Wavelength(s)	1.0
Spacegroup name	P 21 21 2
Unit cell lengths	100.721, 134.215, 72.951
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 * - 2.600
Rwork	0.275
R-free	0.30000 *
Structure solution method	MIR
RMSD bond length	0.007
RMSD bond angle	1.400
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	EPMR
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000 *
High resolution limit [Å]	2.600 *
Rmerge	0.108 *	0.672 *
Total number of observations	140870 *
Number of reflections	28954 *
Completeness [%]	98.7 *	97.7 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.6 *	25 *	PEG2000MME, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 100K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	PEG2000 MME	27 (%)
2	1	drop	ammonium sulfate	250 (mM)
3	1	drop	MP	0.5 (mM)
4	1	drop	HEPES	10 (mM)	pH6.6
5	1	reservoir	PEG2000MME	27 (%)
6	1	reservoir	ammonium sulfate	200 (mM)
7	1	reservoir	Tris	100 (mM)	pH8.6