1NGM
Crystal structure of a yeast Brf1-TBP-DNA ternary complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-07-03 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.100, 0.919425, 0.918969 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 93.610, 152.600, 256.638 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.500 * - 2.950 |
| R-factor | 0.278 |
| Rwork | 0.276 |
| R-free | 0.30800 |
| Structure solution method | MAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.441 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.060 |
| High resolution limit [Å] | 2.950 | 2.950 |
| Rmerge | 0.062 | |
| Total number of observations | 269787 * | |
| Number of reflections | 74859 * | |
| Completeness [%] | 95.5 * | 87 |
| Redundancy | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 4. * | MES, NaCl, BME, glycerol, MgCl2, PEG2000MME, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | MES | ||
| 11 | 1 | 2 | MgCl2 | ||
| 2 | 1 | 1 | NaCl | ||
| 3 | 1 | 1 | BME | ||
| 4 | 1 | 1 | glycerol | ||
| 5 | 1 | 1 | MgCl2 | ||
| 6 | 1 | 1 | PEG2000MME | ||
| 8 | 1 | 2 | NaCl |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | 50 (mM) | pH6.5 | |
| 2 | 1 | reservoir | 100 (mM) | or LiCl | |
| 3 | 1 | reservoir | 10 (mM) | ||
| 4 | 1 | reservoir | 2-mercaptoethanol | 2 (mM) | |
| 5 | 1 | reservoir | glycerol | 25 (%(v/v)) | |
| 6 | 1 | reservoir | PEG2000 MME | 10 (%(v/v)) |
