1NFI
I-KAPPA-B-ALPHA/NF-KAPPA-B COMPLEX
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Detector | BRANDEIS - B4 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 49.094, 91.080, 190.980 |
| Unit cell angles | 90.00, 96.32, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.700 |
| R-factor | 0.222 * |
| Rwork | 0.223 |
| R-free | 0.31500 * |
| Structure solution method | MIR |
| RMSD bond length | 0.010 |
| RMSD bond angle | 23.000 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS (0.5) |
| Refinement software | CNS (0.5) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | |
| High resolution limit [Å] | 2.700 | |
| Rmerge | 0.050 * | 0.206 * |
| Number of reflections | 41990 | |
| Completeness [%] | 92.0 | 83 * |
| Redundancy | 3.7 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 5 | 4, 20 * | pH 5.0 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 20 (mg/ml) | |
| 2 | 1 | drop | ammonium acetate | 75 (mM) | |
| 3 | 1 | drop | dithiothreitol | 10 (mM) | |
| 4 | 1 | drop | ethylenediamine tetraacetic acid | 1 (mM) | |
| 5 | 1 | drop | n-octyl-beta-D-glucopyranoside | 0.05 (%) | |
| 6 | 1 | drop | Tris-HCl | 8 (mM) | |
| 7 | 1 | reservoir | sodium acetate | 25 (mM) | |
| 8 | 1 | reservoir | PEG6000 | 8-10 (%) |
